An essay about the Nipah virus.
Infectious Disease
A sample has been sequenced from an epidemic outbreak from an unknown illness that is going around.
The sequence can be found at the end of this paper.
Part A.
Generate an alignment of related sequences, you must have at least 20 sequences in your alignment.
Generate a phylogenetic tree of the alignment that will enable you to identify the nearest neighbour of the sequence and any related species.
Part B.
Each question should be no more than 1000 words.
- Write a section describing the viral family described, include replication of the virus and transmission.
- What is the history of the virus that you have identified? Include a description of the previous outbreaks.
- Given that you have a patient sick in the hospital, what measures would you take to try and control this new outbreak?
Sequence of unknown etiology:
ATGAGTGATATCTTTGAAGAGGCGGCTAGTTTTAGGAGTTATCAATCTAAGTTAGGGAGAGATGGGAGAG
CTAGTGCAGCAACTGCTACTTTGACAACCAAGATAAGGATATTTGTACCAGCTACTAATAGTCCAGAGCT
CAGATGGGAACTAACATTGTTTGCACTTGATGTGATTAGATCTCCGAGTGCTGCCGAGTCAATGAAAGTT
GGAGCTGCTTTCACACTCATCTCTATGTATTCAGAGAGACCCGGGGCTCTCATTAGAAGTCTCCTCAATG
ACCCAGACATTGAAGCTGTAATAATAGATGTTGGATCAATGGTCAATGGAATACCAGTAATGGAGAGGAG
AGGAGACAAGGCTCAGGAGGAGATGGAAGGCTTGATGAGAATCCTCAAAACTGCTCGGGACAGCAGCAAG
GGAAAGACACCTTTTGTTGACAGCCGAGCTTACGGCCTACGGATAACAGACATGAGCACCCTGGTCTCTG
CAGTTATCACCATCGAGGCCCAGATCTGGATACTGATCGCTAAAGCAGTTACAGCTCCCGACACTGCCGA
GGAAAGTGAAACTAGAAGATGGGCTAAATACGTCCAACAAAAGAGAGTCAATCCGTTCTTTGCTCTAACT
CAGCAATGGCTAACAGAAATGAGGAATCTGCTCTCCCAGAGTCTATCAGTAAGGAAGTTCATGGTTGAGA
TCCTCATAGAAGTCAAGAAAGGAGGATCTGCTAAAGGCAGAGCAGTAGAAATAATCTCAGACATCGGAAA
CTATGTCGAGGAAACTGGTATGGCAGGATTCTTCGCAACCATCAGATTCGGGTTGGAGACAAGGTATCCA
GCACTTGCACTCAACGAATTCCAGAGTGACCTCAACACCATCAAAAGCTTGATGCTACTCTACAGAGAAA
TTGGCCCAAGAGCCCCTTATATGGTGCTTCTTGAAGAATCAATTCAGACTAAATTTGCCCCTGGAGGTTA
CCCATTATTGTGGAGCTTTGCCATGGGTGTGGCTACTACTATTGACAGGTCTATGGGGGCATTGAATATC
AATCGTGGTTATCTTGAGCCTATGTATTTCAGACTAGGCCAAAAATCAGCACGTCACCATGCTGGAGGAA
TTGATCAGAACATGGCAAATAGACTGGGACTAAGTTCAGATCAAGTTGCAGAACTCGCTGCTGCAGTTCA
GGAAACATCAGCAGGAAGACAAGAGAGTAACGTTCAGGCTAGAGAGGCAAAATTTGCTGCAGGAGGTGTG
CTCATTGGAGGCAGTGATCAAGATGTCGATGAAGAGGAAGAACCTATAGAACAGAGTGGCAGACAGTCAG
TTACCTTCAAAAGGGAGATGAGTATTTCATCCCTCGCTGACAGTGTGCCGAGCAGTTCTGTGAGCACATC
CGGTGGGACCAGATTGACCAATTCATTACTAAACCTCAGATCAAGACTGGCTGCAAAAGCAGCAAAAGAA
GCCGCCTCATCCAATGCAACAGATGATCCAGCAATCAGCAACAGAACTCAAGGGGAATCAGAGAAGAAGA
ATAATCAAGACCTCAAACCTGCTCAAAATGACCTTGATTTCGTCAGAGCTGATGTGTGAATGGATAAATT
GGAACTAGTCAATGATGGCCTCAATATTATTGACTTTATTCAGAAGAACCAAAAAGAAATACAGAAGACA
TACGGACGATCAAGCATTCAACAACCCAGCATCAAAGACCGAACAAAAGCCTGGGAAGATTTTCTGCAGT
GCACCAGTGGAGAATCTGAACAAGTTGAGGGGGGAATGTCTAAGGATGATGGAGATGTTGAAAGAAGAAA
CTTGGAGGATCTATCCAGTACTTCTCCCACAGATGGAACTATTGGAAAGAGAGTGTCGAACACCCGTGAC
TGGGCAGAAGGTTCAGATGACATACAACTGGACCCAGTGGTTACAGACGTTGTATACCATGATCATGGAG
GAGAATGTACCGGATATGGATTTACTTCAAGCCCTGAGAGAGGGTGGAGTGATTACGCATCAGGAGCAAA
CAATGGGAATGTATGTCTTGTATCTGATGCAAAGATGCTGTCCTATGCTCCCGAAATTGCAGTTTCTAAA
GAAGATCGGGAAACTGATCTAGTTCATCTTGAGAATAAACTATCTACTACAGGACTGAATCCCACAGCAG
TACCATTCACTCCGAGAAACCTGTCTGATCCTGCAAAAGACTCTCCTGTGATTGCTGAACACTACTACGG
ACTAGGAGTTAAAGAGCAAAACGTTGGCCCTCAGACTAGCAGAAATGTCAATTTGGACAGCATCAAATTG
TACACATCAGATGACGAAGAGGCAGATCAGCTTGAATTCGAAGATGAGTTTGCAGGAAGCTCAAGTGAAG
TAATAGTCGGCATTTCTCCTGAAGATGAAGAGCCTTCAAGTGTCGGCGGAAAACCCAATGAATCCATTGG
ACGTACAATCGAAGGCCAATCAATCCGAGACAGCCTTCAAGCCAAGGACAACAAATCAGCAGATGTACCA
GGAACAGGACCGAAAGATTCAGCAGTGAAGGAAGAACCACCCCAGAAGAGGCTACCTATGTTAGCTGAAG
AATTTGAGTGCTCTGGATCGGAAGACCCAATCATTCGAGAGCTGCTGAAGGAGAACTCACTCATAAATTG
TCAGCAAGGGAAAGATGCTCAGCCTCCATATCATTGGAGCATCGAGAGGTCAATAAGCCCGGATAAGACT
GAGATCGTCAACGGTGCTGTGCAAACTGCTGACAGGCAAAGACCAGGAACTCCGATGCCAAAGTCCCGAG
GTATTCCCATTAAAAAGGGGGGGGACACGAAAAATCCATCTGCTGGGACGGAAAAAATGCCTGGGTCGAA
GAGAGGTGGAACCCGGCCTGTTCGAGGATCACCCCCCCCCCCCCAAAGCAAGAGTGTCAATGCGGAGAAT
GTCCAACTGAATGCTTCCCCTGCGGTGAAGGAAACTGATAAGTCAAAAATAAACCCCCCACACGACAACA
ACTCACTTGATGATAAATACATCATGCCTTCAGATGATTTCTCAAACACTTTCTTCCCGCACGACACTGA
TCGCTTGAATTATCACGCAGATCATTTAGGTGATTATGACCTCGAAACCCTGTGTGAAGAGTCGGTTCTA
ATGGGAGTGATCAACTCTATAAAATTAATTAATCTGGATATGCGCTTAAATCACATTGAAGAACAAGTTA
AAGAGATCCCAAAGATCATCAATAAGCTTGAGTCCATTGACAGAGTTCTGGCCAAGACTAACACCGCACT
CTCAACCATTGAAGGACACCTGGTTTCTATGATGATAATGATACCAGGGAAAGGGAAAGGAGAAAGAAAG
GGGAAAAATAATCCTGAGCTTAAACCAGTGATAGGAAGAGACATTCTAGAGCAGCAATCTCTTTTTTCTT
TCGACAATGTCAAGAATTTCAGAGATGGATCGTTGACAAACGAACCGTATGGAGCAGCTGTACAGTTGAG
AGAAGATCTTATTCTTCCTGAACTTAATTTTGAGGAGACAAATGCATCTCAATTTGTTCCTATGGCAGAT
GATTCATCCAGGGATGTTGTCAAGACATTGATAAGGACTCACATTAAAGATAGAGAGTTGAGATCAGAAC
TGATTGGTTACCTGAATAAAGCGGAAAATGATGAGGAAATTCAGGAGATAGCAAACACTGTCAATGACAT
CATTGACGGTAACATTTGAATGGAGCCGGACATCAAGAGTATTTCAAGTGAGTCAATGGAAGGAGTATCT
GATTTCAGCCCTAGTTCTTGGGAGCATGGTGGGTATCTTGATAAGGTTGAACCAGAAATTGATGAAAATG
GCAGTATGATTCCAAAATACAAGATCTATACCCCAGGAGCTAACGAGAGGAAATACAACAACTACATGTA
CCTTATATGTTACGGCTTTGTTGAAGATGTTGAGAGAACCCCAGAGACAGGGAAACGCAAGAAGATCAGG
ACAATTGCTGCCTACCCTCTGGGTGTTGGTAAGAGTGCCTCTCATCCCCAAGATCTTCTGGAGGAACTCT
GTTCCCTCAAAGTTACTGTGAGGAGAACAGCTGGATCAACTGAGAAAATTGTGTTTGGATCATCTGGCCC
TCTAAATCACCTCGTTCCGTGGAAGAAAGTACTGACTGGTGGTTCAATTTTTAATGCAGTCAAGGTTTGT
CGGAACGTTGATCAGATACAGCTCGACAAGCATCAAGCTCTGAGAATATTTTTTCTCAGTATCACAAAGC
TCAATGATTCTGGAATCTACATGATTCCACGAACCATGCTTGAGTTCAGGAGAAACAATGCCATTGCCTT
CAATCTTCTAGTGTACTTGAAGATTGATGCTGATTTATCCAAAATGGGGATCCAGGGAAGCCTCGATAAA
GATGGCTTCAAGGTTGCCTACTTCATGCTACACTTGGGGAACTTTGTCCGTCGTGCAGGGAAGTATTACT
CTGTTGATTATTGTAGGAGGAAGATTGATAGGATGAAATTGCAGTTTTCACTGGGTTCCATAGGCGGACT
AAGTCTCCACATTAAGATCAATGGTGTAATCAGCAAACGGCTGTTTGCTCAAATGGGATTCCAAAAAAAC
CTTTGTTTCTCCTTGATGGACATCAATCCTTGGCTCAACAGATTGACCTGGAACAACAGTTGTGAGATCA
GCCGAGTAGCAGCTGTGTTGCAGCCTTCTGTTCCAAGAGAGTTCATGATCTATGATGATGTCTTCATTGA
CAATACAGGGAGAATTCTAAAGGGCTAAATGGTAGTTATACTTGACAAGAGATGTTATTCTAATCTTTTA
ATATTGATTTTGATGATCTCGGAGTGTAGTGTTGGGATTCTACATTATGAGAAATTGAGTAAAATTGGAC
TTGTCAAAGGAGTAACAAGAAAATACAAGATTAAAAGCAATCCTCTCACAAAAGACATTGTTATAAAAAT
GATTGCGAATGTGTCGAACATGTCTCAGTGCACAGGGAGTGTCATGGAAAATTATAAAACACGATTAAAC
GGTATCTTAACACCTATAAAGGGAGCGTTAGAGATCTACAAAAACAACACTCATGACCTTGTCGGTGATG
TGAGATTAGCCGGAGTTATAATGGCAGGAGTTGCCATTGGGATTGCAACCGCAGCTCAAATCACTGCAGG
TGTAGCACTATATGAGGCAATGAAGAATGCTGACAACATCAACAAACTCAAAAGCAGCATTGAATCAACT
AATGAAGCTGTCGTTAAACTTCAAGAGACTGCAGAAAAGACAGTCTATGTGCTGACTGCTCTACAGGATT
ACATTAATACTAATTTGGTACCGACAATTGACAAGATAAGCTGCAAACAGACAGAACTCTCACTAGATCT
GGCATTATCAAAGTACCTCTCTGATTTGCTTTTTGTATTTGGCCCCAACCTTCAAGACCCAGTTTCTAAT
TCAATGACTATACAGGCTATATCTCAGGCATTCGGTGGAAATTATGAAACACTGCTAAGAACATTGGGTT
ACGCTACAGAAGACTTTGATGATCTTCTAGAAAGTGACAGCATAACAGGTCAAATCATCTATGTTGATCT
AAGTAGCTACTATATAATTGTCAGGGTTTATTTTCCTATTCTGACTGAAATTCAACAGGCCTATATCCAA
GAGTTGTTACCAGTGAGCTTCAACAATGATAATTCAGAATGGATCAGTATTGTCCCAAATTTCATATTGG
TAAGGAATACATTAATATCAAATATAGAGATTGGATTTTGCCTAATTACAAAGAGGAGCGTGATCTGCAA
CCAAGATTATGCCACACCTATGACCAACAACATGAGAGAATGTTTGACGGGATCGACTGAGAAGTGTCCT
CGAGAGCTGGTTGTTTCATCACATGTTCCCAGATTTGCACTATCTAACGGAGTTCTGTTTGCCAATTGCA
TAAGTGTTACATGCCAGTGTCAAACAACAGGCAGGGCAATCTCACAGTCAGGAGAACAAACTCTGCTGAT
GATTGACAACACCACCTGTCCTACAGCCGTACTCGGTAATGTGATTATCAGCTTAGGGAAATATCTGGGG
TCAGTAAATTATAATTCTGAAGGCATTGCTATCGGTCCTCCAGTCTTTACAGATAAAGTTGATATATCAA
GTCAGAAATCCAGCATGAATCAGTCCTTACAACAGTCTAAGGACTATATCAAAGAGGCTCAACGACTCCT
TGATACTGTTAATCCATCATTAATAAGCATGTTGTCTATGATCATACTGTATGTATTATCGATCGCATCG
TTGTGTATAGGGTTGATTACATTTATCAGTTTTATCATTGTTGAGAAAAAGAGAAACACCTACAGCAGAT
TGGAGGATAGGAGAGTCAGACCTACAAGCAGTGGGGATCTCTACTACATTGGGACATAGATGCCGGCAGA
AAACAAGAAAGTTAGATTCGAAAATACTACTTCAGACAAAGGGAAAAATCCTAGTAAAGTTATTAAGAGC
TACTACGGAACCATGGACATTAAGAAAATAAATGAAGGATTATTGGACAGCAAAATATTAAGTGCTTTCA
ACACAGTAATAGCATTGCTTGGATCTATCGTGATCATAGTGATGAATATAATGATCATCCAAAATTACAC
AAGATCAACAGACAATCAGGCCGTGATCAAAGATGCGTTGCAGGGTATCCAACAGCAGATCAAAGGGCTT
GCTGACAAAATCGGCACAGAGATAGGGCCTAAAGTATCACTGATTGACACATCCAGTACCATTACTATCC
CAGCTAACATTGGGCTGTTAGGTTCAAAGATCAGCCAGTCGACTGCAAGTATAAATGAGAATGTGAATGA
AAAATGCAAATTCACACTGCCTCCCTTGAAAATCCACGAATGTAACATTTCTTGTCCTAACCCACTCCCC
TTTAGAGAGTATAGGCCGCAGACAGAAGGGGTGAGCAATCTGGTAGGATTACCTGATAATATTTGCCTGC
AAAAGACATCTAATCAGATACTGAAGCCAAAGCTGATTTCATACACTTTACCCGTAGTCGGTCAAAGTGG
TACCTGTATCACAGACCCATTGCTGGCTATGGACGAGGGCTATTTTGCATATAGCCACCTGGAAAGAATC
GGATCATGTTCAAGAGGGGTCTCCAAACAAAGAATAATAGGAGTTGGAGAGGTACTAGACAGAGGTGATG
AAGTTCCTTCTTTATTTATGACCAATGTCTGGACCCCACCAAATCCAAACACCGTTTACCACTGTAGTGC
TGTATACAACAATGAATTCTATTATGTACTTTGTGCAGTGTCAACTGTTGGAGACCCTATTCTGAATAGC
ACCTACTGGTCCGGATCTCTAATGATGACCCGTCTAGCTGTGAAACCCAAGAGTAATGGTGGGGGTTACA
ATCAACATCAACTTGCCCTACGAAGTATCGAGAAAGGGAGGTATGATAAAGTTATGCCGTATGGACCTTC
AGGCATCAAACAGGGTGACACCCTGTATTTTCCTGCTGTAGGATTTTTGGTCAGGACAGAGTTTAAATAC
AATGATTCAAATTGTCCCATCACGAAGTGTCAATACAGTAAACCTGAAAATTGCAGGCTATCTATGGGGA
TTAGACCAAACAGCCATTATATCCTTCGATCTGGACTATTAAAATACAATCTATCAGATGGGGAGAACCC
CAAAATTGTATTCATTGAAATATCTGATCAAAGATTATCTATTGGATCTCCTAGCAAAGTCTATGATTCT
TTGGGTCAACCTGTTTTCTACCAAGCGTCATTTTCATGGGATACTATGATTAAATTTGGAGATGTTCAAA
CAGTCAACCCTCTGGTTGTCAATTGGCGTGATAACACGGTAATATCAAGACCCGGGCAATCACAATGCCC
TAGATTCAATACATGTCCAGAGATCTGCTGGGAAGGAGTTTATAATGATGCATTCCTAATTGACAGAATC
AATTGGATAAGCGCGGGTGTATTCCTTGACAGCAATCAGACCGCAGAAAATCCTGTTTTCACTGTATTCA
AAGATAATGAAATACTTTATAGGGCACAACTGGCTTCTGAGGACACCAATGCACAAAAAACAATAACTAA
TTGCTTTCTCTTGAAGAATAAGATTTGGTGCATATCATTGGTTGAGATATATGACACAGGAGACAATGTC
ATAAGACCCAAACTATTCGCGGTTAAGATACCAGAGCAATGTACATAAATGGCCGACGAATTATCAATAT
CCGACATCATTTACCCTGAATGTCATTTGGATAGTCCTATAGTCTCTGGTAAACTAATATCAGCTATTGA
ATATGCTCAATTGAGACACAATCAACCCAGTGATGATAAAAGACTGTCTGAGAATATTAGGTTAAACCTT
CACGGGAAAAGAAAGAGTCTATACATATTAAGACAATCCAAACAGGGTGATTACATTAGAAACAACATAA
AAAACCTAAAGGAATTCATGCATATTGCGTACCCTGAATGCAATAACATTCTATTCTCCATCACATCCCA
AGGCATGACTAGCAAACTTGATAACATCATGAAAAAGTCATTCAAAGCATACAATATCATTAGTAAGAAA
GTAATTGGGATGCTGCAAAATATCACTAGAAATCTCATAACTCAAGATAGAAGAGATGAAATAATTAATA
TACATGAGTGTAGGCGATTAGGGGATTTAGGGAAGAATATGAGTCAATCTAAATGGTATGAGTGTTTTTT
GTTTTGGTTTACTATCAAAACAGAGATGCGAGCAGTGATCAAGAATTCGCAAAAGCCGAAATTCCGTTCA
GATTCATGCATAATACACATGCGAGACAAAAGTACTGAAATAATCCTAAATCCGAATCTTATCTGCATTT
TCAAATCAGACAAAAATGGAAAGAAGTGTTATTATCTTACACCCGAAATGGTTCTAATGTATTGTGATGT
CCTAGAGGGAAGGATGATGATGGAGACAACAGTCAAATCGGATATCAAGTACCAACCTCTAATCTCGAGA
TCCAATGCCCTCTGGGGGCTAATTGATCCCTTGTTCCCTGTCATGGGAAACAGAATTTACAATATAGTGT
CTATGATAGAGCCTTTAGTTCTTGCACTACTCCAACTCAAGGATGAGGCTAGGATCCTGAGGGGTGCATT
TCTGCATCACTGCATAAAGGAAATGCATCAAGAATTGAGTGAGTGTGGTTTTACAGATCAGAAGATTCGG
TCTATGTTTATTGATGATCTTTTATCCATTCTAAATATCGATAATATACATCTGTTGGCAGAGTTCTTTT
CTTTCTTTCGTACGTTTGGCCATCCTATTCTTGAGGCTAAAGTTGCTGCAGAAAAAGTGAGAGAACATAT
GTTGGCAGATAAAGTTCTTGAATATGCCCCTATAATGAAAGCACATGCTATATTCTGCGGGACTATAATA
AATGGGTATAGGGATAGACACGGAGGAGCCTGGCCTCCTCTTTACCTCCCCGCACATGCATCTAAACATA
TAATCCGTTTGAAAAATTCTGGGGAATCTTTGACCATTGATGACTGTGTCAAGAATTGGGAATCATTCTG
TGGGATTCAATTTGATTGTTTCATGGAGCTGAAATTGGACAGTGATCTGAGTATGTATATGAAAGATAAA
GCTTTATCTCCAATCAAAGACGAATGGGACAGTGTATACCCACGTGAAGTGTTGAGCTATACCCCACCGA
AGTCAACCGAGCCAAGAAGATTGGTTGACGTTTTTGTAAATGATGAAAACTTTGATCCATACAACATGCT
GGAATATGTCTTATCCGGTGCTTATCTCGAGGATGAACAATTCAATGTTTCTTATAGCTTGAAGGAGAAA
GAGACGAAGCAAGCTGGACGATTGTTCGCAAAGATGACCTACAAAATGCGTGCATGTCAAGTCATAGCAG
AGGCCCTGATAGCCTCAGGTGTCGGTAAATATTTTAAGGAGAACGGGATGGTTAAGGATGAGCACGAACT
TTTGAAGACACTCTTCCAATTGTCTATTTCCTCAGTTCCTCGAGGGAACAGTCAGGGTAATGATCCTCAA
TCCATCAATAATATAGAAAGAGATTTCCAATACTTTAAAGGGGTCACTACCAATGTGAAAGACAAAAAGA
ATAACTCTTTTAATAAGGTTAAATCTGCTCTCAATAATCCGTGCCAAGCTGACGGAGTCCATCATAACAT
GTCACCCAATACACGAAATCGTTATAAGTGTAGTAATACAAGTAAGTCTTTTCTCGATTATCATACCGAG
TTTAATCCTCACAATCACTATAAATCAGACAATACAGAGGCGGCCGTACTGTCCAGGTATGAGGACAACA
CTGGGACAAAATTTGATACAGTAAGTGCATTTCTTACAACTGATCTTAAGAAATTCTGTCTCAATTGGAG
ATACGAATCAATGGCTATATTTGCTGAACGTCTAGATGAGATATACGGTTTACCTGGATTTTTTAATTGG
ATGCACAAACGACTAGAAAGATCTGTTATCTATGTTGCAGACCCTAATTGCCCCCCTAATATTGACAAAC
ATATGGAACTAGAAAAAACTCCTGAAGATGATATATTCATTCATTATCCTAAAGGCGGTATTGAAGGATA
TAGCCAAAAAACATGGACTATAGCAACTATCCCCTTTTTATTCTTGAGTGCCTATGAGACAAACACGAGG
ATTGCTGCAATTGTCCAAGGAGACAATGAATCAATTGCTATCACTCAAAAAGTTCATCCTAATCTTCCTT
ACAAGGTAAAGAAAGAGATCTGTGCAAAGCAAGCTCAGCTTTATTTTGAAAGGTTAAGGATGAACTTAAG
AGCCCTCGGCCACAATCTTAAAGCTACAGAAACTATCATCAGTACACATCTTTTTATTTATTCGAAGAAA
ATTCATTATGATGGTGCTGTGCTGTCTCAGGCACTCAAATCAATGTCAAGATGTTGCTTTTGGTCAGAGA
CTCTGGTGGATGAAACTAGATCAGCTTGTAGTAACATCAGCACTACAATAGCTAAAGCTATAGAAAATGG
GTTGTCAAGAAATGTCGGCTATTGCATCAATATTTTGAAAGTAATTCAGCAGCTTCTCATATCAACTGAG
TTTAGTATTAACGAGACATTGACACTGGATGTGACATCTCCCATTTCAAATAATTTAGATTGGCTTATAA
CAGCTGCATTAATCCCGGCACCTATTGGAGGATTCAATTACCTTAATTTGTCTAGAATTTTTGTTAGAAA
TATAGGTGATCCGGTTACAGCATCTTTGGCTGATCTTAAGAGAATGATTGATCACAGTATTATGACTGAA
AGCGTATTACAAAAAGTTATGAATCAAGAACCTGGTGATGCGAGTTTCTTGGACTGGGCCAGTGATCCAT
ACTCGGGCAACTTGCCTGACTCACAAAGCATCACTAAAACAATTAAAAATATCACAGCAAGGACTATACT
GAGGAACTCACCGAACCCAATGCTAAAAGGTTTATTTCATGACAAATCTTTTGATGAAGATCTTGAACTA
GCTAGCTTCTTAATGGACAGGAGGGTTATATTACCTAGAGCCGCTCATGAGATACTGGATAATTCATTGA
CAGGTGCCAGAGAGGAAATTGCTGGTTTATTAGATACAACTAAAGGCTTGATCAGATCAGGGCTAAGAAA
GAGTGGACTTCAGCCAAAGTTAGTTTCTAGATTATCTCATCATGATTATAATCAATTTTTAATACTGAAC
AAACTTCTATCAAACAGAAGACAAAATGACTTGATATCATCAAATACTTGCTCAGTTGACTTGGCACGAG
CATTGAGATCTCACATGTGGAGGGAATTAGCGTTAGGTAGAGTAATATACGGTCTTGAGGTACCGGATGC
ACTTGAGGCTATGGTGGGAAGGTATATAACAGGGAGCTTAGAGTGCCAAATTTGTGAGCAGGGAAACACG
ATGTATGGGTGGTTCTTTGTACCTAGGGATTCCCAATTGGATCAGGTAGATAGAGAGCACTCATCAATAA
GAGTACCTTATGTAGGATCAAGTACGGATGAAAGATCGGATATCAAACTAGGGAATGTCAAAAGACCAAC
TAAGGCCTTGCGTTCTGCTATCAGAATTGCGACAGTATATACTTGGGCCTATGGGGACAATGAAGAGTGT
TGGTATGAAGCTTGGTACCTAGCGTCTCAGAGGGTAAACATAGACTTAGATGTATTGAAAGCTATAACCC
CAGTTTCCACTTCAAACAATTTATCCCATAGATTGAGAGATAAATCCACACAATTTAAGTTTGCAGGGAG
TGTACTCAACAGAGTTTCTAGATATGTTAACATAAGCAATGACAATCTAGATTTCAGAATTGAGGGAGAA
AAGGTAGATACGAATCTTATTTATCAACAAGCAATGCTATTAGGGTTATCGGTATTGGAAGGTAAATTCA
GATTGAGATTAGAAACTGATGATTACAACGGGATATATCACTTACACGTAAAGGATAATTGTTGTGTCAA
AGAAGTGGCTGATGTAGGCCAAGTAGACGCTGAGTTGCCTATCCCAGAATATACTGAAGTGGATAACAAT
CATCTTATATATGATCCAGACCCCGTTTCAGAAATAGATTGCAGCCGTCTTTCTAATCAGGAGTCCAAAT
CAAGAGAATTAGACTTTCCTTTATGGTCAACTGAGGAACTTCATGATGTCCTAGCTAAGACTGTTGCTCA
GACCGTTCTTGAGATTATAACAAAGGCTGACAAGGATGTTTTAAAGCAACACCTTGCAATAGACTCTGAC
GATAACATCAACAGCTTAATCACAGAATTTCTAATAGTTGATCCTGAACTGTTTGCACTTTATCTAGGAC
AATCTATATCAATAAAATGGGCCTTTGAAATTCATCATAGGCGTCCTAGAGGAAGACATACTATGGTCGA
CCTATTGTCAGATCTTGTATCAAATACATCAAAGCACACTTACAAAGTGTTGTCAAATGCCTTGTCACAT
CCTAGAGTATTCAAGAGATTTGTAAACTGTGGCTTGCTATTGCCTACACAGGGTCCTTACCTTCATCAAC
AAGATTTTGAAAAGTTGTCTCAAAACCTTCTTGTAACATCTTATATGATTTATCTAATGAACTGGTGTGA
CTTCAAGAAATTCCCCTTTTTAATCGCCGAACAGGATGAAACTGTGATAAGTCTACGAGAGGATATAATA
ACATCCAAACATCTCTGTGTTATAATTGACTTATATGCAAATCACCATAAACCTCCTTGGATAATAGATC
TAAACCCACAAGAAAAAATATGTGTACTGCGTGACTTTATTTCTAAATCTAGGCATGTGGACACGTCCTC
CAGATCATGGAATACTTCTGACCTGGATTTTGTAATATTCTATGCATCTTTGACTTATTTGAGAAGAGGT
ATAATAAAACAATTAAGGATAAGACAAGTTACTGAGGTTATAGATACCACAACAGTGTTAAGGGATAATA
TAATTGTAGAGAATCCTCCTATTAAAACAGGAGTGTTAGACATCAGAGGTTGTATAATATACAATTTAGA
GGAAATCCTGTCTATGAACACAAAATCAGCATCAAAAAAGATCTTTAATCTTAATAGTAGGCCGTCAGTG
GAGAATCATAAATATAGAAGGATAGGTCTCAACTCATCATCTTGTTACAAGGCATTAAATCTATCACCTC
TGATTCAAAGGTATTTGCCGTCGGGAGCTCAAAGGTTGTTTATAGGAGAAGGTTCTGGGAGCATGATGTT
ATTATATCAGTCTACATTGGGGCAATCAATTTCTTTTTACAATTCAGGTATAGATGGAGATTATATACCA
GGTCAAAGAGAACTGAAACTATTTCCCTCTGAATACTCAATTGCTGAGGAAGACCCATCTCTGACGGGGA
AATTGAAAGGACTAGTGGTGCCCCTATTCAATGGAAGACCAGAAACAACATGGATCGGGAATTTAGACTC
CTACGAGTATATCATAAATAGGACAGCGGGGCGAAGTATAGGTCTTGTCCATTCTGACATGGAGTCTGGG
ATTGACAAAAATGTAGAGGAGATACTAGTAGAACATTCCCATCTAATATCTATCGCGATAAATGTTATGA
TGGAGGACGGACTATTAGTATCCAAGATAGCATACACCCCTGGATTCCCAATCTCAAGATTATTTAACAT
GTACAGATCATATTTCGGACTAGTACTGGTGTGTTTCCCAGTATATAGTAATCCAGATTCTACTGAAGTA
TATCTTCTTTGCTTACAGAAGACGGTCAAGACTATTGTTCCCCCGCAAAAAGTCCTTGAGCACTCTAATT
TGAACGATGAAGTCAATGACCAGGGAATAACATCAGTGATTTTTAAAATCAAGAATTCACAGTCTAAGCA
GTTCCACGATGATCTAAAGAAGTACTATCAGATTGACCAACCTTTTTTTGTACCAACTAAAATCACTAGT
GATGAACAAGTACTTCTCCAAGCAGGGCTGAAACTCAATGGGCCAGAAATTCTTAAGAGTGAAATCAGTT
ATGATATCGGTTCAGATATCAATACATTAAGAGACACCATCATAATTATGTTAAATGAGGCTATGAATTA
TTTTGATGACAACAGATCACCTTCACACCACCTAGAACCCTATCCAGTTTTGGAGAGAACTAGAATTAAA
ACAATAATGAATTGTGTGACTAAGAAAGTGATTGTCTACTCACTTATCAAGTTCAAGGACACCAAAAGCT
CAGAACTTTATCACATCAAAAATAACATCAGAAGAAAAGTTCTAATCTTAGATTTCAGATCGAAGCTCAT
GACAAAAACTCTACCTAAAGGGATGCAAGAGAGAAGAGAAAAAAACGGTTTCAAAGAAGTTTGGATAGTA
GATTTATCGAATCGAGAAGTTAAAATCTGGTGGAAGATAATCGGATACATATCTATTATCTGA
